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The Qubit ssDNA Assay Kit is designed specifically for use with the Qubit Fluorometer, but may be used with any fluorometer or fluorescence plate reader. The Qubit® ssDNA Kit is ideal for quantitating single-stranded DNA or oligonucleotides. However, it is not specific for single-stranded DNA. This assay kit will also detect double-stranded DNA and RNA but it will not detect contaminating protein or nucleotides. The assay is designed to be accurate for initial sample concentrations from 50 pg/µL to 200 ng/µL providing an assay range of 1–200 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay. Which product to choose for ssDNA quantitation? • For 1–20 samples: use this Qubit ssDNA Assay Kit with the Qubit Fluorometer • For 20–2000 samples: use the Quant-iT OliGreen ssDNA Assay Kit with microplate reader Notes: 1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers. 2. 500 µL thin-walled PCR tubes are required but not included. |
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