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Thermo Scientific Lambda Exonuclease is a highly processive 5'→3' exodeoxyribonuclease. It selectively digests the 5'-phosphorylated strand of double-stranded DNA. The enzyme exhibits low activity on single-stranded DNA and non-phosphorylated DNA, and has no activity at nicks and limited activity at gaps in DNA. Highlights • Active in Thermo Scientific PCR buffers Applications • Generating single-stranded PCR products for use in: • DNA sequencing • Analysis of DNA single-strand conformation polymorphism (SSCP) • Rolling circle amplification • Producing single-stranded DNA from double-stranded DNA fragments • Cloning of PCR products Note Use of this enzyme in certain applications may be covered by patents and may require a license. |
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